Detailed information of TA system TA122755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 950847..951325
• Replicon: chromosome
• Accession: NZ_CP038428
• Organism: Escherichia coli O157:H7 strain 611

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: E3149_RS04475
• Protein ID: WP_001303876.1
• Coordinates: 950847..951134 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: E3149_RS04480
• Protein ID: WP_000536233.1
• Coordinates: 951134..951325 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3149_RS04445	945996..948467	-	2472	WP_000034474.1	exonuclease	-
E3149_RS04450	948561..948752	-	192	WP_001098307.1	DUF1482 family protein	-
E3149_RS04455	948749..948937	-	189	WP_000413705.1	cell division inhibition protein DicB	-
E3149_RS04460	949511..949696	+	186	WP_001133046.1	hypothetical protein	-
E3149_RS04465	949883..950272	-	390	WP_000394511.1	hypothetical protein	-
E3149_RS04470	950414..950569	-	156	WP_000379575.1	DUF1391 family protein	-
E3149_RS04475	950847..951134	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3149_RS04480	951134..951325	-	192	WP_000536233.1	hypothetical protein	Antitoxin
E3149_RS04485	951353..951754	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
E3149_RS04490	951863..952135	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
E3149_RS04495	952119..952544	+	426	WP_000693855.1	Rha family transcriptional regulator	-
E3149_RS04500	952751..953206	-	456	WP_000273724.1	hypothetical protein	-
E3149_RS04505	953285..954376	+	1092	WP_001205823.1	hypothetical protein	-
E3149_RS04510	954383..955129	+	747	WP_000788751.1	ATP-binding protein	-
E3149_RS04515	955151..955921	+	771	WP_000450992.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T122755 WP_001303876.1 NZ_CP038428:c951134-950847 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T122755 NZ_CP038428:c951134-950847 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT122755 WP_000536233.1 NZ_CP038428:c951325-951134 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT122755 NZ_CP038428:c951325-951134 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA