Detailed information of TA system TA122754

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 837784..838579
• Replicon: chromosome
• Accession: NZ_CP038428
• Organism: Escherichia coli O157:H7 strain 611

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: E3149_RS03980
• Protein ID: WP_000854914.1
• Coordinates: 838205..838579 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0B0VS41
• Locus tag: E3149_RS03975
• Protein ID: WP_001280954.1
• Coordinates: 837784..838158 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3149_RS03945	833139..834044	+	906	WP_000203541.1	chemotaxis protein	-
E3149_RS03950	834041..835111	+	1071	WP_171880038.1	patatin-like phospholipase family protein	-
E3149_RS03955	835451..836269	+	819	WP_001234682.1	DUF945 domain-containing protein	-
E3149_RS03960	836360..836845	+	486	WP_000214398.1	antirestriction protein	-
E3149_RS03965	836861..837337	+	477	WP_001186738.1	RadC family protein	-
E3149_RS03970	837400..837621	+	222	WP_001220314.1	DUF987 domain-containing protein	-
E3149_RS03975	837784..838158	+	375	WP_001280954.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E3149_RS03980	838205..838579	+	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
E3149_RS03985	838576..839067	+	492	WP_000976853.1	hypothetical protein	-
E3149_RS03990	839079..839276	+	198	WP_000839282.1	DUF957 domain-containing protein	-
E3149_RS03995	839361..840203	+	843	WP_001695143.1	DUF4942 domain-containing protein	-
E3149_RS04005	840694..840912	-	219	WP_001040187.1	translation initiation factor IF-1	-
E3149_RS04010	841197..841901	-	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	iroB	808879..851721	42842

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T122754 WP_000854914.1 NZ_CP038428:838205-838579 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T122754 NZ_CP038428:838205-838579 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13718.51 Da
• Isoelectric Point: 6.6249

>AT122754 WP_001280954.1 NZ_CP038428:837784-838158 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT122754 NZ_CP038428:837784-838158 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A0B0VS41