Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 72152..72711 Replicon plasmid p2571-1
Accession NZ_CP038426
Organism Escherichia coli O157:H7 strain 2571

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag E3150_RS28145 Protein ID WP_000421248.1
Coordinates 72436..72711 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag E3150_RS28140 Protein ID WP_001178089.1
Coordinates 72152..72436 (+) Length 95 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3150_RS28100 67344..67904 + 561 WP_000840472.1 fertility inhibition protein FinO -
E3150_RS28105 68037..68249 + 213 WP_001302189.1 ANR family transcriptional regulator -
E3150_RS28110 68494..68955 + 462 WP_001233853.1 thermonuclease family protein -
E3150_RS28115 69001..69210 + 210 WP_001302200.1 hemolysin expression modulator Hha -
E3150_RS28120 69248..69586 + 339 WP_000766796.1 hypothetical protein -
E3150_RS28125 69826..70080 + 255 WP_000083831.1 replication regulatory protein RepA -
E3150_RS28130 70316..70390 + 75 WP_001370046.1 RepA leader peptide Tap -
E3150_RS28135 70383..71240 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
E3150_RS28140 72152..72436 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
E3150_RS28145 72436..72711 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3150_RS28150 72806..73012 + 207 WP_001105064.1 hypothetical protein -
E3150_RS28155 73112..73327 - 216 Protein_79 transposase -
E3150_RS28160 73371..73937 - 567 Protein_80 IS3-like element IS600 family transposase -
E3150_RS28165 73937..74353 - 417 Protein_81 transposase zinc-binding domain-containing protein -
E3150_RS28170 74353..74538 - 186 WP_001302179.1 hypothetical protein -
E3150_RS28175 74715..76925 + 2211 WP_000592771.1 catalase/peroxidase KatP -
E3150_RS28180 76969..77358 + 390 WP_001172748.1 cytochrome b562 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..92986 92986


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T122751 WP_000421248.1 NZ_CP038426:72436-72711 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T122751 NZ_CP038426:72436-72711 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT122751 WP_001178089.1 NZ_CP038426:72152-72436 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT122751 NZ_CP038426:72152-72436 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0


Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References