Detailed information of TA system TA122740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3668485..3668963
• Replicon: chromosome
• Accession: NZ_CP038425
• Organism: Escherichia coli O157:H7 strain 2571

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: E3150_RS19070
• Protein ID: WP_001303876.1
• Coordinates: 3668676..3668963 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: E3150_RS19065
• Protein ID: WP_000536233.1
• Coordinates: 3668485..3668676 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3150_RS19030	3663889..3664659	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E3150_RS19035	3664681..3665427	-	747	WP_000788751.1	ATP-binding protein	-
E3150_RS19040	3665434..3666525	-	1092	WP_072143748.1	DNA-binding protein	-
E3150_RS19045	3666604..3667059	+	456	WP_000273724.1	hypothetical protein	-
E3150_RS19050	3667266..3667691	-	426	WP_000693855.1	Rha family transcriptional regulator	-
E3150_RS19055	3667675..3667947	-	273	WP_032362955.1	helix-turn-helix domain-containing protein	-
E3150_RS19060	3668056..3668457	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
E3150_RS19065	3668485..3668676	+	192	WP_000536233.1	hypothetical protein	Antitoxin
E3150_RS19070	3668676..3668963	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3150_RS19075	3669241..3669396	+	156	WP_000379575.1	DUF1391 family protein	-
E3150_RS19080	3669538..3669927	+	390	WP_000394511.1	hypothetical protein	-
E3150_RS19085	3670114..3670299	-	186	WP_001133046.1	hypothetical protein	-
E3150_RS19090	3670873..3671061	+	189	WP_000413705.1	cell division inhibition protein DicB	-
E3150_RS19095	3671058..3671249	+	192	WP_001098307.1	DUF1482 family protein	-
E3150_RS19100	3671343..3673814	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T122740 WP_001303876.1 NZ_CP038425:3668676-3668963 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T122740 NZ_CP038425:3668676-3668963 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT122740 WP_000536233.1 NZ_CP038425:3668485-3668676 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT122740 NZ_CP038425:3668485-3668676 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA