Detailed information of TA system TA122737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2609432..2609903
• Replicon: chromosome
• Accession: NZ_CP038425
• Organism: Escherichia coli O157:H7 strain 2571

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E3150_RS13200
• Protein ID: WP_001303511.1
• Coordinates: 2609432..2609710 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E3150_RS13205
• Protein ID: WP_001302048.1
• Coordinates: 2609712..2609903 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3150_RS13160	2604438..2604622	-	185	Protein_2573	DUF3596 domain-containing protein	-
E3150_RS13165	2604657..2604908	-	252	WP_000005552.1	excisionase family protein	-
E3150_RS13170	2604981..2607452	-	2472	WP_000048458.1	exonuclease	-
E3150_RS13175	2607545..2607736	-	192	WP_001090200.1	DUF1482 family protein	-
E3150_RS13180	2607733..2607921	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E3150_RS13185	2608322..2608486	+	165	WP_001331716.1	hypothetical protein	-
E3150_RS13190	2608490..2608708	-	219	WP_001171930.1	hypothetical protein	-
E3150_RS13195	2608780..2609079	-	300	WP_001240334.1	hypothetical protein	-
E3150_RS13200	2609432..2609710	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3150_RS13205	2609712..2609903	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E3150_RS13210	2609924..2610295	-	372	WP_001169686.1	hypothetical protein	-
E3150_RS13215	2610393..2610695	+	303	WP_000172738.1	transcriptional regulator	-
E3150_RS13220	2610692..2611117	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E3150_RS13225	2611140..2612102	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E3150_RS13230	2612109..2612849	+	741	WP_000788938.1	ATP-binding protein	-
E3150_RS13235	2612875..2613644	+	770	Protein_2588	DUF1627 domain-containing protein	-
E3150_RS13240	2613660..2614055	+	396	WP_001118159.1	DUF977 family protein	-
E3150_RS13245	2614112..2614696	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	espX7/nleL / espN / espK	2602271..2715952	113681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T122737 WP_001303511.1 NZ_CP038425:c2609710-2609432 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T122737 NZ_CP038425:c2609710-2609432 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT122737 WP_001302048.1 NZ_CP038425:c2609903-2609712 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT122737 NZ_CP038425:c2609903-2609712 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• AlphaFold DB: A0A829CTP0