Detailed information of TA system TA122700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2560618..2561089
• Replicon: chromosome
• Accession: NZ_CP038423
• Organism: Escherichia coli O157:H7 strain 7409

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E3151_RS12865
• Protein ID: WP_001303511.1
• Coordinates: 2560618..2560896 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E3151_RS12870
• Protein ID: WP_001302048.1
• Coordinates: 2560898..2561089 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3151_RS12825	2555624..2555808	-	185	Protein_2505	DUF3596 domain-containing protein	-
E3151_RS12830	2555843..2556094	-	252	WP_000005552.1	excisionase family protein	-
E3151_RS12835	2556167..2558638	-	2472	WP_000048458.1	exonuclease	-
E3151_RS12840	2558731..2558922	-	192	WP_001090200.1	DUF1482 family protein	-
E3151_RS12845	2558919..2559107	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E3151_RS12850	2559508..2559672	+	165	WP_001331716.1	hypothetical protein	-
E3151_RS12855	2559676..2559894	-	219	WP_001171930.1	hypothetical protein	-
E3151_RS12860	2559966..2560265	-	300	WP_001240334.1	hypothetical protein	-
E3151_RS12865	2560618..2560896	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3151_RS12870	2560898..2561089	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E3151_RS12875	2561110..2561481	-	372	WP_001169686.1	hypothetical protein	-
E3151_RS12880	2561579..2561881	+	303	WP_000172738.1	transcriptional regulator	-
E3151_RS12885	2561878..2562303	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E3151_RS12890	2562326..2563288	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
E3151_RS12895	2563295..2564035	+	741	WP_000788936.1	ATP-binding protein	-
E3151_RS12900	2564061..2564830	+	770	Protein_2520	DUF1627 domain-containing protein	-
E3151_RS12905	2564846..2565241	+	396	WP_001118159.1	DUF977 family protein	-
E3151_RS12910	2565298..2565882	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2552331..2598431	46100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T122700 WP_001303511.1 NZ_CP038423:c2560896-2560618 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T122700 NZ_CP038423:c2560896-2560618 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT122700 WP_001302048.1 NZ_CP038423:c2561089-2560898 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT122700 NZ_CP038423:c2561089-2560898 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0