Detailed information of TA system TA122673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4160877..4161495
• Replicon: chromosome
• Accession: NZ_CP038421
• Organism: Escherichia coli O157:H7 strain 7636

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: E3152_RS20885
• Protein ID: WP_171881827.1
• Coordinates: 4161277..4161495 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: E3152_RS20880
• Protein ID: WP_000344800.1
• Coordinates: 4160877..4161251 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3152_RS20870	4155966..4157159	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
E3152_RS20875	4157182..4160331	+	3150	WP_001132452.1	multidrug efflux RND transporter permease subunit	-
E3152_RS20880	4160877..4161251	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
E3152_RS20885	4161277..4161495	+	219	WP_171881827.1	hemolysin expression modulator Hha	Toxin
E3152_RS20890	4161667..4162218	+	552	WP_000102553.1	maltose O-acetyltransferase	-
E3152_RS20895	4162334..4162804	+	471	WP_000136192.1	YlaC family protein	-
E3152_RS20900	4162968..4164518	+	1551	WP_001301851.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
E3152_RS20905	4164560..4164913	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
E3152_RS20915	4165292..4165603	+	312	WP_000409911.1	MGMT family protein	-
E3152_RS20920	4165636..4166208	-	573	WP_000779831.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8617.96 Da
• Isoelectric Point: 8.9008

>T122673 WP_171881827.1 NZ_CP038421:4161277-4161495 [Escherichia coli O157:H7]
MSEKSLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T122673 NZ_CP038421:4161277-4161495 [Escherichia coli O157:H7]
ATGTCCGAAAAATCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT122673 WP_000344800.1 NZ_CP038421:4160877-4161251 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT122673 NZ_CP038421:4160877-4161251 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5