Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5092588..5093085 Replicon chromosome
Accession NZ_CP038419
Organism Escherichia coli O157:H7 strain 2-6-2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag E4U89_RS25495 Protein ID WP_001173343.1
Coordinates 5092588..5092761 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag E4U89_RS25500 Protein ID WP_000398619.1
Coordinates 5092789..5093085 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U89_RS25485 5089258..5090715 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
E4U89_RS25490 5090952..5092469 + 1518 WP_001295074.1 lysine--tRNA ligase -
E4U89_RS25495 5092588..5092761 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
E4U89_RS25500 5092789..5093085 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
E4U89_RS25505 5093312..5093584 - 273 WP_000405647.1 N-acetyltransferase -
E4U89_RS25510 5093596..5093826 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
E4U89_RS25515 5094007..5095638 + 1632 WP_001216477.1 sensor histidine kinase -
E4U89_RS25520 5095635..5096354 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T122643 WP_001173343.1 NZ_CP038419:c5092761-5092588 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T122643 NZ_CP038419:c5092761-5092588 [Escherichia coli O157:H7]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT122643 WP_000398619.1 NZ_CP038419:c5093085-5092789 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT122643 NZ_CP038419:c5093085-5092789 [Escherichia coli O157:H7]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References