Detailed information of TA system TA122634

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3650501..3651296
• Replicon: chromosome
• Accession: NZ_CP038419
• Organism: Escherichia coli O157:H7 strain 2-6-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: E4U89_RS18740
• Protein ID: WP_000854914.1
• Coordinates: 3650501..3650875 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0B0VS41
• Locus tag: E4U89_RS18745
• Protein ID: WP_001280954.1
• Coordinates: 3650922..3651296 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U89_RS18710	3647179..3647883	+	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-
E4U89_RS18715	3648168..3648386	+	219	WP_001040187.1	translation initiation factor IF-1	-
E4U89_RS18725	3648877..3649719	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
E4U89_RS18730	3649804..3650001	-	198	WP_000839282.1	DUF957 domain-containing protein	-
E4U89_RS18735	3650013..3650504	-	492	WP_000976857.1	hypothetical protein	-
E4U89_RS18740	3650501..3650875	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
E4U89_RS18745	3650922..3651296	-	375	WP_001280954.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E4U89_RS18750	3651459..3651680	-	222	WP_000692323.1	DUF987 domain-containing protein	-
E4U89_RS18755	3651743..3652219	-	477	WP_001186738.1	RadC family protein	-
E4U89_RS18760	3652235..3652720	-	486	WP_000214398.1	antirestriction protein	-
E4U89_RS18765	3652811..3653629	-	819	WP_001234682.1	DUF945 domain-containing protein	-
E4U89_RS18770	3653969..3655039	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
E4U89_RS18775	3655036..3655941	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T122634 WP_000854914.1 NZ_CP038419:c3650875-3650501 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T122634 NZ_CP038419:c3650875-3650501 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13718.51 Da
• Isoelectric Point: 6.6249

>AT122634 WP_001280954.1 NZ_CP038419:c3651296-3650922 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT122634 NZ_CP038419:c3651296-3650922 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A0B0VS41