Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2785275..2785913 Replicon chromosome
Accession NZ_CP038419
Organism Escherichia coli O157:H7 strain 2-6-2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag E4U89_RS14010 Protein ID WP_000813794.1
Coordinates 2785275..2785451 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag E4U89_RS14015 Protein ID WP_001270286.1
Coordinates 2785497..2785913 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U89_RS13990 2780897..2782108 - 1212 WP_071525382.1 BenE family transporter YdcO -
E4U89_RS13995 2782161..2782697 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
E4U89_RS14000 2782770..2784731 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
E4U89_RS14005 2784823..2785053 - 231 WP_000494244.1 YncJ family protein -
E4U89_RS14010 2785275..2785451 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
E4U89_RS14015 2785497..2785913 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
E4U89_RS14020 2785992..2787398 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
E4U89_RS14025 2787643..2788788 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
E4U89_RS14030 2788806..2789819 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
E4U89_RS14035 2789820..2790761 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T122627 WP_000813794.1 NZ_CP038419:2785275-2785451 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T122627 NZ_CP038419:2785275-2785451 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT122627 WP_001270286.1 NZ_CP038419:2785497-2785913 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT122627 NZ_CP038419:2785497-2785913 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References