Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 73338..73897 | Replicon | plasmid pGM351-1 |
| Accession | NZ_CP038418 | ||
| Organism | Escherichia coli O157:H7 strain 3-5-1 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | E4U90_RS27950 | Protein ID | WP_000421248.1 |
| Coordinates | 73622..73897 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | E4U90_RS27945 | Protein ID | WP_001178089.1 |
| Coordinates | 73338..73622 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E4U90_RS27905 | 68531..69091 | + | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| E4U90_RS27910 | 69224..69436 | + | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| E4U90_RS27915 | 69681..70142 | + | 462 | WP_001233856.1 | thermonuclease family protein | - |
| E4U90_RS27920 | 70188..70397 | + | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| E4U90_RS27925 | 70435..70773 | + | 339 | WP_000766796.1 | hypothetical protein | - |
| E4U90_RS27930 | 71013..71267 | + | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| E4U90_RS27935 | 71503..71577 | + | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| E4U90_RS27940 | 71570..72427 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| E4U90_RS27945 | 73338..73622 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| E4U90_RS27950 | 73622..73897 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| E4U90_RS27955 | 73992..74198 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| E4U90_RS27960 | 74298..74513 | - | 216 | Protein_80 | transposase | - |
| E4U90_RS27965 | 74557..75123 | - | 567 | Protein_81 | IS3-like element IS600 family transposase | - |
| E4U90_RS27970 | 75123..75539 | - | 417 | Protein_82 | transposase zinc-binding domain-containing protein | - |
| E4U90_RS27975 | 75539..75724 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| E4U90_RS27980 | 75901..78111 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| E4U90_RS27985 | 78155..78544 | + | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP | 1..94184 | 94184 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T122608 WP_000421248.1 NZ_CP038418:73622-73897 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T122608 NZ_CP038418:73622-73897 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT122608 WP_001178089.1 NZ_CP038418:73338-73622 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT122608 NZ_CP038418:73338-73622 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |