Detailed information of TA system TA122600

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4918130..4918725
• Replicon: chromosome
• Accession: NZ_CP038416
• Organism: Escherichia coli O157:H7 strain 3-5-1

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: E4U90_RS24395
• Protein ID: WP_000239579.1
• Coordinates: 4918130..4918480 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: E4U90_RS24400
• Protein ID: WP_001223210.1
• Coordinates: 4918474..4918725 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U90_RS24375	4913576..4914598	-	1023	WP_001301928.1	ABC transporter permease	-
E4U90_RS24380	4914612..4916114	-	1503	Protein_4774	sugar ABC transporter ATP-binding protein	-
E4U90_RS24385	4916254..4917210	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
E4U90_RS24390	4917520..4918050	+	531	WP_000055075.1	inorganic diphosphatase	-
E4U90_RS24395	4918130..4918480	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
E4U90_RS24400	4918474..4918725	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
E4U90_RS24405	4918937..4919278	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
E4U90_RS24410	4919281..4923060	-	3780	WP_000060931.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T122600 WP_000239579.1 NZ_CP038416:c4918480-4918130 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T122600 NZ_CP038416:c4918480-4918130 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT122600 WP_001223210.1 NZ_CP038416:c4918725-4918474 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT122600 NZ_CP038416:c4918725-4918474 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8