Detailed information of TA system TA122593

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3717512..3718217
• Replicon: chromosome
• Accession: NZ_CP038416
• Organism: Escherichia coli O157:H7 strain 3-5-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: E4U90_RS18965
• Protein ID: WP_000539519.1
• Coordinates: 3717512..3717898 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E4U90_RS18970
• Protein ID: WP_001280945.1
• Coordinates: 3717888..3718217 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U90_RS18945	3713516..3714142	+	627	WP_001295292.1	glutathione S-transferase GstB	-
E4U90_RS18950	3714139..3715254	-	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
E4U90_RS18955	3715365..3715748	-	384	WP_000497137.1	biofilm formation regulator BssR	-
E4U90_RS18960	3715961..3717286	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
E4U90_RS18965	3717512..3717898	+	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U90_RS18970	3717888..3718217	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
E4U90_RS18975	3718287..3719615	-	1329	WP_000086905.1	GGDEF domain-containing protein	-
E4U90_RS18980	3719623..3721971	-	2349	WP_000950320.1	EAL domain-containing protein	-
E4U90_RS18985	3722149..3723060	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T122593 WP_000539519.1 NZ_CP038416:3717512-3717898 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T122593 NZ_CP038416:3717512-3717898 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT122593 WP_001280945.1 NZ_CP038416:3717888-3718217 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT122593 NZ_CP038416:3717888-3718217 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA