Detailed information of TA system TA122585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2584937..2585408
• Replicon: chromosome
• Accession: NZ_CP038416
• Organism: Escherichia coli O157:H7 strain 3-5-1

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U90_RS13000
• Protein ID: WP_001303511.1
• Coordinates: 2584937..2585215 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U90_RS13005
• Protein ID: WP_001302048.1
• Coordinates: 2585217..2585408 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U90_RS12960	2579944..2580128	-	185	Protein_2533	DUF3596 domain-containing protein	-
E4U90_RS12965	2580163..2580414	-	252	WP_000005552.1	excisionase family protein	-
E4U90_RS12970	2580487..2582958	-	2472	WP_000048458.1	exonuclease	-
E4U90_RS12975	2583051..2583242	-	192	WP_001090200.1	DUF1482 family protein	-
E4U90_RS12980	2583239..2583427	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U90_RS12985	2583828..2583992	+	165	WP_001331716.1	hypothetical protein	-
E4U90_RS12990	2583996..2584214	-	219	WP_001171930.1	hypothetical protein	-
E4U90_RS12995	2584286..2584585	-	300	WP_001240334.1	hypothetical protein	-
E4U90_RS13000	2584937..2585215	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U90_RS13005	2585217..2585408	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U90_RS13010	2585429..2585800	-	372	WP_001169686.1	hypothetical protein	-
E4U90_RS13015	2585898..2586200	+	303	WP_000172738.1	transcriptional regulator	-
E4U90_RS13020	2586197..2586622	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U90_RS13025	2586645..2587607	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E4U90_RS13030	2587614..2588354	+	741	WP_000788938.1	ATP-binding protein	-
E4U90_RS13035	2588380..2589149	+	770	Protein_2548	DUF1627 domain-containing protein	-
E4U90_RS13040	2589165..2589560	+	396	WP_001118159.1	DUF977 family protein	-
E4U90_RS13045	2589617..2590201	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2576651..2674812	98161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T122585 WP_001303511.1 NZ_CP038416:c2585215-2584937 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T122585 NZ_CP038416:c2585215-2584937 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT122585 WP_001302048.1 NZ_CP038416:c2585408-2585217 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT122585 NZ_CP038416:c2585408-2585217 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0