Detailed information of TA system TA122564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4983503..4984098
• Replicon: chromosome
• Accession: NZ_CP038414
• Organism: Escherichia coli O157:H7 strain 17B6-2

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: E4U86_RS24705
• Protein ID: WP_000239579.1
• Coordinates: 4983503..4983853 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: E4U86_RS24710
• Protein ID: WP_001223210.1
• Coordinates: 4983847..4984098 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U86_RS24685	4978949..4979971	-	1023	WP_001301928.1	ABC transporter permease	-
E4U86_RS24690	4979985..4981487	-	1503	WP_171878336.1	ATP-binding cassette domain-containing protein	-
E4U86_RS24695	4981627..4982583	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
E4U86_RS24700	4982893..4983423	+	531	WP_000055075.1	inorganic diphosphatase	-
E4U86_RS24705	4983503..4983853	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
E4U86_RS24710	4983847..4984098	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
E4U86_RS24715	4984310..4984651	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
E4U86_RS24720	4984654..4988433	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T122564 WP_000239579.1 NZ_CP038414:c4983853-4983503 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T122564 NZ_CP038414:c4983853-4983503 [Escherichia coli O157:H7]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT122564 WP_001223210.1 NZ_CP038414:c4984098-4983847 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT122564 NZ_CP038414:c4984098-4983847 [Escherichia coli O157:H7]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGTGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8