Detailed information of TA system TA122555

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3647732..3648527
• Replicon: chromosome
• Accession: NZ_CP038414
• Organism: Escherichia coli O157:H7 strain 17B6-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: E4U86_RS18655
• Protein ID: WP_000854914.1
• Coordinates: 3647732..3648106 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0B0VS41
• Locus tag: E4U86_RS18660
• Protein ID: WP_001280954.1
• Coordinates: 3648153..3648527 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U86_RS18625	3644410..3645114	+	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-
E4U86_RS18630	3645399..3645617	+	219	WP_001040187.1	translation initiation factor IF-1	-
E4U86_RS18640	3646108..3646950	-	843	WP_001280472.1	DUF4942 domain-containing protein	-
E4U86_RS18645	3647035..3647232	-	198	WP_000839282.1	DUF957 domain-containing protein	-
E4U86_RS18650	3647244..3647735	-	492	WP_000976853.1	hypothetical protein	-
E4U86_RS18655	3647732..3648106	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
E4U86_RS18660	3648153..3648527	-	375	WP_001280954.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E4U86_RS18665	3648690..3648911	-	222	WP_001220314.1	DUF987 domain-containing protein	-
E4U86_RS18670	3648974..3649450	-	477	WP_001186738.1	RadC family protein	-
E4U86_RS18675	3649466..3649951	-	486	WP_032305785.1	antirestriction protein	-
E4U86_RS18680	3650042..3650860	-	819	WP_001234682.1	DUF945 domain-containing protein	-
E4U86_RS18685	3651200..3652270	-	1071	WP_000102643.1	patatin-like phospholipase family protein	-
E4U86_RS18690	3652267..3653172	-	906	WP_021497799.1	yjcZ-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T122555 WP_000854914.1 NZ_CP038414:c3648106-3647732 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T122555 NZ_CP038414:c3648106-3647732 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13718.51 Da
• Isoelectric Point: 6.6249

>AT122555 WP_001280954.1 NZ_CP038414:c3648527-3648153 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT122555 NZ_CP038414:c3648527-3648153 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A0B0VS41