Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 87945..88470 Replicon plasmid p493-89-1
Accession NZ_CP038413
Organism Escherichia coli O157:H7 strain 493/89

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag E3172_RS27560 Protein ID WP_001159868.1
Coordinates 87945..88250 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag E3172_RS27565 Protein ID WP_000813634.1
Coordinates 88252..88470 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3172_RS27545 83908..85074 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
E3172_RS27550 85662..86417 - 756 WP_000852146.1 replication initiation protein RepE -
E3172_RS27555 87138..87944 - 807 WP_000016989.1 site-specific integrase -
E3172_RS27560 87945..88250 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
E3172_RS27565 88252..88470 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E3172_RS27570 88929..89612 + 684 WP_010891291.1 DNA-binding protein -
E3172_RS27575 89609..90229 + 621 WP_001248529.1 hypothetical protein -
E3172_RS27580 90226..91014 + 789 WP_000708303.1 DUF2971 domain-containing protein -
E3172_RS27585 91050..91790 + 741 WP_000933099.1 abortive infection family protein -
E3172_RS27590 91949..92890 - 942 WP_000554874.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - papC / hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE 1..121214 121214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T122530 WP_001159868.1 NZ_CP038413:c88250-87945 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T122530 NZ_CP038413:c88250-87945 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT122530 WP_000813634.1 NZ_CP038413:c88470-88252 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT122530 NZ_CP038413:c88470-88252 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References