Detailed information of TA system TA122525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 39195..39820
• Replicon: plasmid p493-89-1
• Accession: NZ_CP038413
• Organism: Escherichia coli O157:H7 strain 493/89

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: E3172_RS27230
• Protein ID: WP_000911317.1
• Coordinates: 39422..39820 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: E3172_RS27225
• Protein ID: WP_000450532.1
• Coordinates: 39195..39422 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3172_RS27225	39195..39422	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
E3172_RS27230	39422..39820	+	399	WP_000911317.1	tRNA(fMet)-specific endonuclease VapC	Toxin
E3172_RS27235	39829..42018	-	2190	WP_024201115.1	type IV conjugative transfer system coupling protein TraD	-
E3172_RS27240	42271..43002	-	732	WP_000782452.1	complement resistance protein TraT	-
E3172_RS27245	43051..43536	-	486	WP_000605866.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papC / hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE	1..121214	121214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T122525 WP_000911317.1 NZ_CP038413:39422-39820 [Escherichia coli O157:H7]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T122525 NZ_CP038413:39422-39820 [Escherichia coli O157:H7]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT122525 WP_000450532.1 NZ_CP038413:39195-39422 [Escherichia coli O157:H7]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT122525 NZ_CP038413:39195-39422 [Escherichia coli O157:H7]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5