Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1093501..1094084 Replicon chromosome
Accession NZ_CP038412
Organism Escherichia coli O157:H7 strain 493/89

Toxin (Protein)


Gene name mazF Uniprot ID C3SX57
Locus tag E3172_RS05300 Protein ID WP_000254740.1
Coordinates 1093749..1094084 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag E3172_RS05295 Protein ID WP_000581937.1
Coordinates 1093501..1093749 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3172_RS05285 1089840..1091141 + 1302 WP_000046824.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
E3172_RS05290 1091189..1093423 + 2235 WP_000226815.1 GTP pyrophosphokinase -
E3172_RS05295 1093501..1093749 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
E3172_RS05300 1093749..1094084 + 336 WP_000254740.1 endoribonuclease MazF Toxin
E3172_RS05305 1094155..1094946 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
E3172_RS05310 1095174..1096811 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
E3172_RS05315 1096899..1098197 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12197.18 Da        Isoelectric Point: 8.7218

>T122497 WP_000254740.1 NZ_CP038412:1093749-1094084 [Escherichia coli O157:H7]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKRTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T122497 NZ_CP038412:1093749-1094084 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAAGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT122497 WP_000581937.1 NZ_CP038412:1093501-1093749 [Escherichia coli O157:H7]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT122497 NZ_CP038412:1093501-1093749 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References