Detailed information of TA system TA122415

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3706038..3706833
• Replicon: chromosome
• Accession: NZ_CP038402
• Organism: Escherichia coli O157:H7 strain BB24-1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: E4U87_RS19065
• Protein ID: WP_000854914.1
• Coordinates: 3706038..3706412 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9LM11
• Locus tag: E4U87_RS19070
• Protein ID: WP_001280953.1
• Coordinates: 3706459..3706833 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U87_RS19035	3702716..3703420	+	705	WP_001241680.1	leucyl/phenylalanyl-tRNA--protein transferase	-
E4U87_RS19040	3703705..3703923	+	219	WP_001040187.1	translation initiation factor IF-1	-
E4U87_RS19050	3704414..3705256	-	843	WP_001280481.1	DUF4942 domain-containing protein	-
E4U87_RS19055	3705341..3705538	-	198	WP_000839282.1	DUF957 domain-containing protein	-
E4U87_RS19060	3705550..3706041	-	492	WP_000976857.1	hypothetical protein	-
E4U87_RS19065	3706038..3706412	-	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
E4U87_RS19070	3706459..3706833	-	375	WP_001280953.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E4U87_RS19075	3706996..3707217	-	222	WP_000692323.1	DUF987 domain-containing protein	-
E4U87_RS19080	3707280..3707756	-	477	WP_001186738.1	RadC family protein	-
E4U87_RS19085	3707772..3708257	-	486	WP_000214398.1	antirestriction protein	-
E4U87_RS19090	3708348..3709166	-	819	WP_001234682.1	DUF945 domain-containing protein	-
E4U87_RS19095	3709506..3710576	-	1071	WP_000102669.1	patatin-like phospholipase family protein	-
E4U87_RS19100	3710573..3711478	-	906	WP_000203541.1	chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T122415 WP_000854914.1 NZ_CP038402:c3706412-3706038 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T122415 NZ_CP038402:c3706412-3706038 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13734.56 Da
• Isoelectric Point: 6.6249

>AT122415 WP_001280953.1 NZ_CP038402:c3706833-3706459 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPALATTS

Nucleotide

• Length: 375 bp

>AT122415 NZ_CP038402:c3706833-3706459 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACTCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A7U9LM11