Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 370439..371070 Replicon chromosome
Accession NZ_CP038402
Organism Escherichia coli O157:H7 strain BB24-1

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag E4U87_RS01705 Protein ID WP_001259385.1
Coordinates 370439..370714 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E4U87_RS01710 Protein ID WP_000593555.1
Coordinates 370711..371070 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U87_RS01690 365443..366510 + 1068 WP_000361458.1 HlyD family secretion protein -
E4U87_RS01695 366507..369242 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
E4U87_RS01700 369242..370366 + 1125 WP_001301659.1 ABC transporter permease -
E4U87_RS01705 370439..370714 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
E4U87_RS01710 370711..371070 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4U87_RS01715 371120..371980 - 861 WP_001131788.1 class II aldolase -
E4U87_RS01720 372012..372281 - 270 WP_000933060.1 HPr family phosphocarrier protein -
E4U87_RS01725 372271..373779 - 1509 WP_001301773.1 carbohydrate kinase -
E4U87_RS01730 373772..375130 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
E4U87_RS01735 375207..375488 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
E4U87_RS01740 375485..375958 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 361583..371070 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T122389 WP_001259385.1 NZ_CP038402:370439-370714 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T122389 NZ_CP038402:370439-370714 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT122389 WP_000593555.1 NZ_CP038402:370711-371070 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT122389 NZ_CP038402:370711-371070 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References