Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3999006..3999624 Replicon chromosome
Accession NZ_CP038394
Organism Escherichia coli O157:H7 strain DEC5A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E3155_RS19740 Protein ID WP_001291435.1
Coordinates 3999406..3999624 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E3155_RS19735 Protein ID WP_000344800.1
Coordinates 3999006..3999380 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3155_RS19725 3994095..3995288 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E3155_RS19730 3995311..3998460 + 3150 WP_001132468.1 multidrug efflux RND transporter permease subunit -
E3155_RS19735 3999006..3999380 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E3155_RS19740 3999406..3999624 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E3155_RS19745 3999796..4000347 + 552 WP_000102564.1 maltose O-acetyltransferase -
E3155_RS19750 4000463..4000933 + 471 WP_000136192.1 YlaC family protein -
E3155_RS19755 4001097..4002647 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E3155_RS19760 4002689..4003042 - 354 WP_000878140.1 DUF1428 domain-containing protein -
E3155_RS19770 4003421..4003732 + 312 WP_000409911.1 MGMT family protein -
E3155_RS19775 4003765..4004337 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T122339 WP_001291435.1 NZ_CP038394:3999406-3999624 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T122339 NZ_CP038394:3999406-3999624 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT122339 WP_000344800.1 NZ_CP038394:3999006-3999380 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT122339 NZ_CP038394:3999006-3999380 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References