Detailed information of TA system TA122338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3490063..3490768
• Replicon: chromosome
• Accession: NZ_CP038394
• Organism: Escherichia coli O157:H7 strain DEC5A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q8X3N0
• Locus tag: E3155_RS17275
• Protein ID: WP_000539519.1
• Coordinates: 3490063..3490449 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E3155_RS17280
• Protein ID: WP_001280945.1
• Coordinates: 3490439..3490768 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3155_RS17255	3486067..3486693	+	627	WP_001295292.1	glutathione S-transferase GstB	-
E3155_RS17260	3486690..3487805	-	1116	WP_000554959.1	aldose sugar dehydrogenase YliI	-
E3155_RS17265	3487916..3488299	-	384	WP_000497137.1	biofilm formation regulator BssR	-
E3155_RS17270	3488512..3489837	+	1326	WP_000049364.1	30S ribosomal protein S12 methylthiotransferase RimO	-
E3155_RS17275	3490063..3490449	+	387	WP_000539519.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3155_RS17280	3490439..3490768	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
E3155_RS17285	3490838..3492166	-	1329	WP_000086905.1	GGDEF domain-containing protein	-
E3155_RS17290	3492174..3494522	-	2349	WP_000950334.1	EAL domain-containing protein	-
E3155_RS17295	3494700..3495611	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14279.42 Da
• Isoelectric Point: 9.9296

>T122338 WP_000539519.1 NZ_CP038394:3490063-3490449 [Escherichia coli O157:H7]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T122338 NZ_CP038394:3490063-3490449 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT122338 WP_001280945.1 NZ_CP038394:3490439-3490768 [Escherichia coli O157:H7]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT122338 NZ_CP038394:3490439-3490768 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA