Detailed information of TA system TA122323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1141722..1142305
• Replicon: chromosome
• Accession: NZ_CP038394
• Organism: Escherichia coli O157:H7 strain DEC5A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: E3155_RS05540
• Protein ID: WP_000254738.1
• Coordinates: 1141970..1142305 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: E3155_RS05535
• Protein ID: WP_000581937.1
• Coordinates: 1141722..1141970 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3155_RS05525	1138061..1139362	+	1302	WP_000046824.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
E3155_RS05530	1139410..1141644	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
E3155_RS05535	1141722..1141970	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3155_RS05540	1141970..1142305	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
E3155_RS05545	1142376..1143167	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
E3155_RS05550	1143395..1145032	+	1638	WP_000210874.1	CTP synthase (glutamine hydrolyzing)	-
E3155_RS05555	1145120..1146418	+	1299	WP_000036720.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• Antitoxin PemI-like (2-82)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T122323 WP_000254738.1 NZ_CP038394:1141970-1142305 [Escherichia coli O157:H7]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T122323 NZ_CP038394:1141970-1142305 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9323.68 Da
• Isoelectric Point: 4.4665

>AT122323 WP_000581937.1 NZ_CP038394:1141722-1141970 [Escherichia coli O157:H7]
VIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT122323 NZ_CP038394:1141722-1141970 [Escherichia coli O157:H7]
GTGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• AlphaFold DB: A0A7U9LMB4