Detailed information of TA system TA122311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 25372..26147
• Replicon: plasmid pDEC5B-4
• Accession: NZ_CP038392
• Organism: Escherichia coli O157:H7 strain DEC5B

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8D9PPA8
• Locus tag: E3156_RS25975
• Protein ID: WP_000405244.1
• Coordinates: 25372..25854 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: E3156_RS25980
• Protein ID: WP_001346256.1
• Coordinates: 25845..26147 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3156_RS25955	22330..22587	-	258	WP_001418855.1	ribbon-helix-helix domain-containing protein	-
E3156_RS25960	23648..24505	-	858	WP_000131019.1	incFII family plasmid replication initiator RepA	-
E3156_RS25965	24498..24572	-	75	WP_001365705.1	RepA leader peptide Tap	-
E3156_RS25970	24805..25062	-	258	WP_000078114.1	replication regulatory protein RepA	-
E3156_RS25975	25372..25854	-	483	WP_000405244.1	GNAT family N-acetyltransferase	Toxin
E3156_RS25980	25845..26147	-	303	WP_001346256.1	DUF1778 domain-containing protein	Antitoxin
E3156_RS25985	26469..26618	-	150	WP_064717443.1	type I toxin-antitoxin system Hok family toxin	-
-	26663..26722	+	60	NuclAT_0	-	-
-	26663..26722	+	60	NuclAT_0	-	-
-	26663..26722	+	60	NuclAT_0	-	-
-	26663..26722	+	60	NuclAT_0	-	-
E3156_RS25990	26981..27112	-	132	WP_001418856.1	hypothetical protein	-
E3156_RS25995	27297..27509	-	213	WP_032320915.1	hypothetical protein	-
E3156_RS26000	27639..28199	-	561	WP_096837425.1	fertility inhibition protein FinO	-
E3156_RS26005	28254..28994	-	741	WP_000206157.1	conjugal transfer pilus acetylase TraX	-
E3156_RS26010	29014..30720	-	1707	Protein_41	conjugative transfer relaxase/helicase TraI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	espT / espC	1..75328	75328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-99)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17659.41 Da
• Isoelectric Point: 7.1036

>T122311 WP_000405244.1 NZ_CP038392:c25854-25372 [Escherichia coli O157:H7]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLHGRAMKNQMLNASRTFVICLEGTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T122311 NZ_CP038392:c25854-25372 [Escherichia coli O157:H7]
ATGGAAATAAATGTCACCGCACCGGCATTACTGACGGATGAGCATATACTTCAGCCATTTGATTGTGGGAATGAAGTGTT
AAGTAACTGGTTACATGGTCGCGCTATGAAAAACCAGATGCTCAATGCTTCCCGCACGTTCGTCATTTGCCTGGAGGGTA
CTTTACGTGTTGTGGGGTACTACTCATTAGCTACCGGTTCCGTCACTCATGCCGAACTTGGGCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTTCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTACTCAGGGGCATGGTTTTGGTAAATG
GCTGCTGAGTGATGCCATTCATCGGGTTGTCAATCTGGCTGATCAGGTTGGTATTAAGGCTGTTATGGTTCATGCAATTG
ATGATGATGCCAGAGCGTTTTATGAGCGTTTTGGTTTTGTCCAGTCAGTTGTGGCACCAAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11602.19 Da
• Isoelectric Point: 4.4569

>AT122311 WP_001346256.1 NZ_CP038392:c26147-25845 [Escherichia coli O157:H7]
MEVVMPTTASIRKTPRENQINIRATDEERAVIDYAASLVNKNRTDFIIELAYQEAKNIILDQRLFVLDDECYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT122311 NZ_CP038392:c26147-25845 [Escherichia coli O157:H7]
ATGGAGGTTGTTATGCCTACTACTGCAAGCATCCGTAAAACACCGCGAGAAAATCAGATTAACATCCGGGCAACGGATGA
GGAACGAGCCGTTATTGATTATGCCGCTAGCCTGGTCAATAAGAACCGTACTGATTTCATCATTGAGCTGGCTTACCAGG
AAGCTAAAAACATCATCCTGGATCAACGTTTATTTGTGCTGGATGATGAATGTTATGATTCATTTATTACGCAACTTGAA
GCTCCTGTGCAGAATGCTGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA