Detailed information of TA system TA122301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2611081..2611552
• Replicon: chromosome
• Accession: NZ_CP038389
• Organism: Escherichia coli O157:H7 strain DEC5B

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E3156_RS12920
• Protein ID: WP_001303511.1
• Coordinates: 2611081..2611359 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E3156_RS12925
• Protein ID: WP_001302048.1
• Coordinates: 2611361..2611552 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3156_RS12885	2606306..2606557	-	252	WP_000005552.1	excisionase family protein	-
E3156_RS12890	2606630..2609101	-	2472	WP_000048438.1	exonuclease	-
E3156_RS12895	2609194..2609385	-	192	WP_001090200.1	DUF1482 family protein	-
E3156_RS12900	2609382..2609570	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E3156_RS12905	2609971..2610135	+	165	WP_001331716.1	hypothetical protein	-
E3156_RS12910	2610139..2610357	-	219	WP_001171904.1	hypothetical protein	-
E3156_RS12915	2610429..2610728	-	300	WP_001240334.1	hypothetical protein	-
E3156_RS12920	2611081..2611359	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3156_RS12925	2611361..2611552	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E3156_RS12930	2611573..2611944	-	372	WP_001169686.1	hypothetical protein	-
E3156_RS12935	2612042..2612344	+	303	WP_000172738.1	transcriptional regulator	-
E3156_RS12940	2612341..2612766	+	426	WP_000693946.1	Rha family transcriptional regulator	-
E3156_RS12945	2612789..2613751	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E3156_RS12950	2613758..2614498	+	741	Protein_2535	ATP-binding protein	-
E3156_RS12955	2614524..2615294	+	771	WP_000450876.1	DUF1627 domain-containing protein	-
E3156_RS12960	2615310..2615705	+	396	WP_001118159.1	DUF977 family protein	-
E3156_RS12965	2615762..2616346	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2600956..2649555	48599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T122301 WP_001303511.1 NZ_CP038389:c2611359-2611081 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T122301 NZ_CP038389:c2611359-2611081 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT122301 WP_001302048.1 NZ_CP038389:c2611552-2611361 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT122301 NZ_CP038389:c2611552-2611361 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0