Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 48434..48981 Replicon plasmid pDEC5D-3
Accession NZ_CP038388
Organism Escherichia coli O157:H7 strain DEC5D

Toxin (Protein)


Gene name parE Uniprot ID A0A896ZGX1
Locus tag E3157_RS25260 Protein ID WP_000969997.1
Coordinates 48434..48715 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A7U8WKC6
Locus tag E3157_RS25265 Protein ID WP_000079946.1
Coordinates 48712..48981 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3157_RS25235 44260..44664 + 405 WP_000839179.1 IS66 family insertion sequence hypothetical protein -
E3157_RS25240 44661..45008 + 348 WP_000612550.1 IS66 family insertion sequence element accessory protein TnpB -
E3157_RS25245 45057..46595 + 1539 WP_000099187.1 IS66-like element ISEc22 family transposase -
E3157_RS25250 47010..47597 - 588 WP_001418189.1 serine acetyltransferase -
E3157_RS25255 47897..48238 + 342 WP_000578011.1 hypothetical protein -
E3157_RS25260 48434..48715 - 282 WP_000969997.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3157_RS25265 48712..48981 - 270 WP_000079946.1 hypothetical protein Antitoxin
E3157_RS25270 49036..49194 - 159 WP_000829588.1 hypothetical protein -
E3157_RS25275 49896..50003 - 108 Protein_60 incFII family plasmid replication initiator RepA -
E3157_RS25280 50026..50274 - 249 WP_000083841.1 replication regulatory protein RepA -
E3157_RS25285 50503..50796 - 294 Protein_62 hypothetical protein -
E3157_RS25290 51095..51310 - 216 Protein_63 IS3 family transposase -
E3157_RS25295 51385..51786 - 402 WP_001222321.1 GspS family T2SS pilot lipoprotein variant EptO -
E3157_RS25300 51880..52713 - 834 WP_000088803.1 A24 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - nleA/espI 1..57827 57827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10879.54 Da        Isoelectric Point: 8.4615

>T122284 WP_000969997.1 NZ_CP038388:c48715-48434 [Escherichia coli O157:H7]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YVLRVLHTAQKWP

Download         Length: 282 bp

>T122284 NZ_CP038388:c48715-48434 [Escherichia coli O157:H7]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTC
GTGTGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATGTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10210.55 Da        Isoelectric Point: 8.8735

>AT122284 WP_000079946.1 NZ_CP038388:c48981-48712 [Escherichia coli O157:H7]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT122284 NZ_CP038388:c48981-48712 [Escherichia coli O157:H7]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGTGGTGATGTGCTTCCACATGACATGGTCGAATCCTCCGCGACTGCATGGCGGGAT
GAAATGAGCAGAAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A896ZGX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U8WKC6

References