Detailed information of TA system TA122283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 13946..14481
• Replicon: plasmid pDEC5D-3
• Accession: NZ_CP038388
• Organism: Escherichia coli O157:H7 strain DEC5D

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: E3157_RS25085
• Protein ID: WP_001270421.1
• Coordinates: 14194..14481 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0M724
• Locus tag: E3157_RS25080
• Protein ID: WP_001132893.1
• Coordinates: 13946..14197 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3157_RS25055	9485..9790	-	306	WP_032163448.1	hypothetical protein	-
E3157_RS25060	9794..10210	-	417	Protein_17	DUF1281 domain-containing protein	-
E3157_RS25065	10241..11252	+	1012	Protein_18	DUF4113 domain-containing protein	-
E3157_RS25070	11393..12355	-	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
E3157_RS25075	12352..13557	-	1206	WP_000817637.1	AAA family ATPase	-
E3157_RS25080	13946..14197	+	252	WP_001132893.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3157_RS25085	14194..14481	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3157_RS25090	14483..14888	+	406	Protein_23	DDE-type integrase/transposase/recombinase	-
E3157_RS25095	15534..16511	+	978	WP_000361616.1	RepB family plasmid replication initiator protein	-
E3157_RS25100	16795..17535	-	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
E3157_RS25105	17659..17799	-	141	Protein_26	serine/threonine protein kinase	-
E3157_RS25110	17850..18011	+	162	Protein_27	epoxyqueuosine reductase QueH	-
E3157_RS25115	18322..18696	-	375	WP_001368887.1	hypothetical protein	-
E3157_RS25120	18899..19078	+	180	WP_000526857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	nleA/espI	1..57827	57827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T122283 WP_001270421.1 NZ_CP038388:14194-14481 [Escherichia coli O157:H7]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T122283 NZ_CP038388:14194-14481 [Escherichia coli O157:H7]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9192.50 Da
• Isoelectric Point: 4.2108

>AT122283 WP_001132893.1 NZ_CP038388:13946-14197 [Escherichia coli O157:H7]
MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT122283 NZ_CP038388:13946-14197 [Escherichia coli O157:H7]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V0M724