Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 29101..29648 Replicon plasmid pDEC5E-3
Accession NZ_CP038384
Organism Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)


Gene name parE Uniprot ID A0A896ZGX1
Locus tag E3158_RS27330 Protein ID WP_000969997.1
Coordinates 29101..29382 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A7U8WKC6
Locus tag E3158_RS27335 Protein ID WP_000079946.1
Coordinates 29379..29648 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3158_RS27310 25610..26449 - 840 Protein_30 hypothetical protein -
E3158_RS27315 26802..27395 + 594 WP_000517696.1 hypothetical protein -
E3158_RS27320 27677..28264 - 588 WP_001418189.1 serine acetyltransferase -
E3158_RS27325 28564..28905 + 342 WP_000578011.1 hypothetical protein -
E3158_RS27330 29101..29382 - 282 WP_000969997.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3158_RS27335 29379..29648 - 270 WP_000079946.1 hypothetical protein Antitoxin
E3158_RS27340 29703..29861 - 159 WP_000829588.1 hypothetical protein -
E3158_RS27345 30563..30670 - 108 Protein_37 incFII family plasmid replication initiator RepA -
E3158_RS27350 30693..30941 - 249 WP_000083841.1 replication regulatory protein RepA -
E3158_RS27355 31170..31451 - 282 Protein_39 hypothetical protein -
E3158_RS27360 31750..31965 - 216 Protein_40 IS3 family transposase -
E3158_RS27365 32040..32441 - 402 WP_001222321.1 GspS family T2SS pilot lipoprotein variant EptO -
E3158_RS27370 32535..33368 - 834 WP_000088800.1 A24 family peptidase -
E3158_RS27375 33420..33935 - 516 WP_000998852.1 type II secretion system protein M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul1 / qacE / ant(3'')-Ia exeG / exeE / stcE 1..69832 69832


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10879.54 Da        Isoelectric Point: 8.4615

>T122253 WP_000969997.1 NZ_CP038384:c29382-29101 [Escherichia coli O157:H7]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YVLRVLHTAQKWP

Download         Length: 282 bp

>T122253 NZ_CP038384:c29382-29101 [Escherichia coli O157:H7]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTC
GTGTGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATGTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10210.55 Da        Isoelectric Point: 8.8735

>AT122253 WP_000079946.1 NZ_CP038384:c29648-29379 [Escherichia coli O157:H7]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT122253 NZ_CP038384:c29648-29379 [Escherichia coli O157:H7]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGTGGTGATGTGCTTCCACATGACATGGTCGAATCCTCCGCGACTGCATGGCGGGAT
GAAATGAGCAGAAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A896ZGX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U8WKC6

References