Detailed information of TA system TA122249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4823399..4824219
• Replicon: chromosome
• Accession: NZ_CP038383
• Organism: Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: E3158_RS23555
• Protein ID: WP_001054379.1
• Coordinates: 4823399..4823656 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: E3158_RS23560
• Protein ID: WP_000123957.1
• Coordinates: 4823668..4824219 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3158_RS23535	4818686..4819792	+	1107	WP_001420160.1	N-acetylneuraminate epimerase	-
E3158_RS23540	4819856..4820836	+	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
E3158_RS23545	4821419..4822645	-	1227	Protein_4615	DNA helicase	-
E3158_RS23550	4822723..4823022	+	300	WP_001332034.1	GNAT family N-acetyltransferase	-
E3158_RS23555	4823399..4823656	+	258	WP_001054379.1	YjhX family toxin	Toxin
E3158_RS23560	4823668..4824219	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
E3158_RS23565	4824271..4825017	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
E3158_RS23570	4825145..4825405	+	261	WP_000077645.1	hypothetical protein	-
E3158_RS23575	4825804..4826925	+	1122	WP_000010833.1	M42 family metallopeptidase	-
E3158_RS23580	4826922..4827200	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
E3158_RS23585	4827212..4828525	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T122249 WP_001054379.1 NZ_CP038383:4823399-4823656 [Escherichia coli O157:H7]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T122249 NZ_CP038383:4823399-4823656 [Escherichia coli O157:H7]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT122249 WP_000123957.1 NZ_CP038383:4823668-4824219 [Escherichia coli O157:H7]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT122249 NZ_CP038383:4823668-4824219 [Escherichia coli O157:H7]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030