Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4787264..4787840 Replicon chromosome
Accession NZ_CP038383
Organism Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag E3158_RS23380 Protein ID WP_001297643.1
Coordinates 4787553..4787840 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag E3158_RS23375 Protein ID WP_000063160.1
Coordinates 4787264..4787566 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3158_RS23360 4783860..4786010 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
E3158_RS23365 4786060..4786263 + 204 WP_000467859.1 YbdD/YjiX family protein -
E3158_RS23370 4786274..4787230 + 957 WP_001297640.1 GTPase -
E3158_RS23375 4787264..4787566 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
E3158_RS23380 4787553..4787840 - 288 WP_001297643.1 BrnT family toxin Toxin
E3158_RS23385 4788039..4788736 - 698 Protein_4583 IS1 family transposase -
E3158_RS23390 4789051..4790283 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
E3158_RS23395 4790324..4791604 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4788039..4788332 293


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T122248 WP_001297643.1 NZ_CP038383:c4787840-4787553 [Escherichia coli O157:H7]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T122248 NZ_CP038383:c4787840-4787553 [Escherichia coli O157:H7]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT122248 WP_000063160.1 NZ_CP038383:c4787566-4787264 [Escherichia coli O157:H7]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT122248 NZ_CP038383:c4787566-4787264 [Escherichia coli O157:H7]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References