Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4182349..4182967 Replicon chromosome
Accession NZ_CP038383
Organism Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E3158_RS20560 Protein ID WP_001291435.1
Coordinates 4182749..4182967 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E3158_RS20555 Protein ID WP_000344800.1
Coordinates 4182349..4182723 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3158_RS20545 4177438..4178631 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E3158_RS20550 4178654..4181803 + 3150 WP_001132468.1 multidrug efflux RND transporter permease subunit -
E3158_RS20555 4182349..4182723 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E3158_RS20560 4182749..4182967 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E3158_RS20565 4183139..4183690 + 552 WP_000102564.1 maltose O-acetyltransferase -
E3158_RS20570 4183806..4184276 + 471 WP_000136192.1 YlaC family protein -
E3158_RS20575 4184440..4185990 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E3158_RS20580 4186032..4186385 - 354 WP_000878140.1 DUF1428 domain-containing protein -
E3158_RS20590 4186764..4187075 + 312 WP_000409911.1 MGMT family protein -
E3158_RS20595 4187108..4187680 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T122244 WP_001291435.1 NZ_CP038383:4182749-4182967 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T122244 NZ_CP038383:4182749-4182967 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT122244 WP_000344800.1 NZ_CP038383:4182349-4182723 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT122244 NZ_CP038383:4182349-4182723 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References