Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 3288321..3288792 Replicon chromosome
Accession NZ_CP038383
Organism Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E3158_RS16335 Protein ID WP_001303511.1
Coordinates 3288514..3288792 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E3158_RS16330 Protein ID WP_001302048.1
Coordinates 3288321..3288512 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3158_RS16295 3284174..3284563 - 390 Protein_3195 DUF977 family protein -
E3158_RS16300 3284579..3285349 - 771 WP_000450876.1 DUF1627 domain-containing protein -
E3158_RS16305 3285375..3286115 - 741 Protein_3197 ATP-binding protein -
E3158_RS16310 3286122..3287084 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E3158_RS16315 3287107..3287532 - 426 WP_000693943.1 Rha family transcriptional regulator -
E3158_RS16320 3287529..3287831 - 303 WP_000172738.1 transcriptional regulator -
E3158_RS16325 3287929..3288300 + 372 WP_001169687.1 hypothetical protein -
E3158_RS16330 3288321..3288512 + 192 WP_001302048.1 hypothetical protein Antitoxin
E3158_RS16335 3288514..3288792 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3158_RS16340 3289145..3289444 + 300 WP_001240334.1 hypothetical protein -
E3158_RS16345 3289516..3289734 + 219 WP_001171930.1 hypothetical protein -
E3158_RS16350 3289738..3289902 - 165 WP_001331716.1 hypothetical protein -
E3158_RS16355 3290141..3291592 + 1452 WP_077631791.1 exonuclease -
E3158_RS16360 3291654..3291923 + 270 WP_000003742.1 excisionase -
E3158_RS16365 3291892..3293009 + 1118 Protein_3209 phage integrase Arm DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espJ / espFu/tccP 3245218..3298891 53673
- flank IS/Tn - - 3282601..3284145 1544


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T122241 WP_001303511.1 NZ_CP038383:3288514-3288792 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T122241 NZ_CP038383:3288514-3288792 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT122241 WP_001302048.1 NZ_CP038383:3288321-3288512 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT122241 NZ_CP038383:3288321-3288512 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References