Detailed information of TA system TA122231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2629613..2630251
• Replicon: chromosome
• Accession: NZ_CP038383
• Organism: Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: E3158_RS12860
• Protein ID: WP_000813794.1
• Coordinates: 2629613..2629789 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E3158_RS12865
• Protein ID: WP_001270286.1
• Coordinates: 2629835..2630251 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3158_RS12840	2625235..2626446	-	1212	WP_071525382.1	BenE family transporter YdcO	-
E3158_RS12845	2626499..2627035	+	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
E3158_RS12850	2627108..2629069	+	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
E3158_RS12855	2629161..2629391	-	231	WP_000494244.1	YncJ family protein	-
E3158_RS12860	2629613..2629789	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
E3158_RS12865	2629835..2630251	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
E3158_RS12870	2630330..2631736	+	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
E3158_RS12875	2631981..2633126	+	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
E3158_RS12880	2633144..2634157	+	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
E3158_RS12885	2634158..2635099	+	942	WP_001251310.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T122231 WP_000813794.1 NZ_CP038383:2629613-2629789 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T122231 NZ_CP038383:2629613-2629789 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT122231 WP_001270286.1 NZ_CP038383:2629835-2630251 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT122231 NZ_CP038383:2629835-2630251 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB