122229

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1165019..1165602 Replicon chromosome
Accession NZ_CP038383
Organism Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag E3158_RS05625 Protein ID WP_000254738.1
Coordinates 1165267..1165602 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag E3158_RS05620 Protein ID WP_000581937.1
Coordinates 1165019..1165267 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3158_RS05600 1160188..1160885 + 698 Protein_1100 IS1 family transposase -
E3158_RS05605 1160882..1161301 - 420 Protein_1101 two-component sensor histidine kinase BarA -
E3158_RS05610 1161358..1162659 + 1302 WP_000046824.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
E3158_RS05615 1162707..1164941 + 2235 WP_000226815.1 GTP pyrophosphokinase -
E3158_RS05620 1165019..1165267 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
E3158_RS05625 1165267..1165602 + 336 WP_000254738.1 endoribonuclease MazF Toxin
E3158_RS05630 1165673..1166464 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
E3158_RS05635 1166692..1168329 + 1638 WP_000210874.1 CTP synthase (glutamine hydrolyzing) -
E3158_RS05640 1168417..1169715 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T122229 WP_000254738.1 NZ_CP038383:1165267-1165602 [Escherichia coli O157:H7]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T122229 NZ_CP038383:1165267-1165602 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT122229 WP_000581937.1 NZ_CP038383:1165019-1165267 [Escherichia coli O157:H7]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT122229 NZ_CP038383:1165019-1165267 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LMB4

References