Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 910143..910978 Replicon chromosome
Accession NZ_CP038383
Organism Escherichia coli O157:H7 strain DEC5E

Toxin (Protein)


Gene name yeeV Uniprot ID S1PMM7
Locus tag E3158_RS04390 Protein ID WP_000854720.1
Coordinates 910143..910520 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1P8X0
Locus tag E3158_RS04395 Protein ID WP_001539669.1
Coordinates 910610..910978 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3158_RS04355 905366..905986 + 621 Protein_857 IS66-like element ISEc8 family transposase -
E3158_RS04360 906103..907368 - 1266 WP_001218882.1 integrase arm-type DNA-binding domain-containing protein -
E3158_RS04365 907832..908158 - 327 WP_000779482.1 type II toxin-antitoxin system RelE/ParE family toxin -
E3158_RS04370 908155..908418 - 264 WP_001143297.1 type II toxin-antitoxin system ParD family antitoxin -
E3158_RS04375 908490..909356 - 867 WP_001290179.1 DUF4942 domain-containing protein -
E3158_RS04380 909449..909646 - 198 WP_000839294.1 DUF957 domain-containing protein -
E3158_RS04385 909658..910146 - 489 WP_000777672.1 hypothetical protein -
E3158_RS04390 910143..910520 - 378 WP_000854720.1 TA system toxin CbtA family protein Toxin
E3158_RS04395 910610..910978 - 369 WP_001539669.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
E3158_RS04400 911058..911279 - 222 WP_000692349.1 DUF987 domain-containing protein -
E3158_RS04405 911366..911842 - 477 WP_001186788.1 RadC family protein -
E3158_RS04410 911858..912343 - 486 WP_000849596.1 antirestriction protein -
E3158_RS04415 912398..913216 - 819 WP_001234642.1 DUF945 domain-containing protein -
E3158_RS04420 913316..913549 - 234 WP_001119719.1 DUF905 family protein -
E3158_RS04425 913635..914039 + 405 WP_000839179.1 IS66 family insertion sequence hypothetical protein -
E3158_RS04430 914036..914383 + 348 WP_000612617.1 IS66 family insertion sequence element accessory protein TnpB -
E3158_RS04435 914432..915970 + 1539 WP_000099145.1 IS66-like element ISEc22 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14089.02 Da        Isoelectric Point: 7.9087

>T122227 WP_000854720.1 NZ_CP038383:c910520-910143 [Escherichia coli O157:H7]
MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T122227 NZ_CP038383:c910520-910143 [Escherichia coli O157:H7]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13614.39 Da        Isoelectric Point: 5.9550

>AT122227 WP_001539669.1 NZ_CP038383:c910978-910610 [Escherichia coli O157:H7]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSN
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT122227 NZ_CP038383:c910978-910610 [Escherichia coli O157:H7]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCATGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAAT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FW05

Antitoxin

Source ID Structure
AlphaFold DB A0A829FYK7

References