Detailed information of TA system TA122219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 25845..26346
• Replicon: plasmid pE32511-2
• Accession: NZ_CP038381
• Organism: Escherichia coli O157:H7 strain E32511

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: Q9F571
• Locus tag: E3170_RS27185
• Protein ID: WP_000702246.1
• Coordinates: 26086..26346 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: E3170_RS27180
• Protein ID: WP_001132407.1
• Coordinates: 25845..26096 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3170_RS27140	21040..21741	+	702	WP_000280995.1	hypothetical protein	-
E3170_RS27145	21788..22387	+	600	WP_000091422.1	hypothetical protein	-
E3170_RS27150	22407..22733	+	327	WP_000206697.1	hypothetical protein	-
E3170_RS27155	22864..23301	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
E3170_RS27160	23326..23508	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
E3170_RS27165	24042..24224	+	183	WP_001481829.1	hypothetical protein	-
E3170_RS27170	24331..24597	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
E3170_RS27175	25017..25295	-	279	WP_000545937.1	hypothetical protein	-
E3170_RS27180	25845..26096	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
E3170_RS27185	26086..26346	-	261	WP_000702246.1	hypothetical protein	Toxin
E3170_RS27190	26397..26621	-	225	WP_000814384.1	hypothetical protein	-
E3170_RS27195	26719..26928	-	210	WP_000866037.1	hypothetical protein	-
E3170_RS27200	26970..27293	-	324	WP_000427402.1	hypothetical protein	-
E3170_RS27205	27874..28203	-	330	WP_000855118.1	hypothetical protein	-
E3170_RS27210	28801..29466	+	666	WP_000532148.1	P-loop NTPase	-
E3170_RS27215	29659..29829	+	171	WP_000186151.1	hypothetical protein	-
E3170_RS27220	29847..30443	-	597	WP_000125166.1	hypothetical protein	-
E3170_RS27225	30593..30754	-	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
E3170_RS27230	30947..31108	-	162	WP_001128311.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..58509	58509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9891.42 Da
• Isoelectric Point: 10.0665

>T122219 WP_000702246.1 NZ_CP038381:c26346-26086 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIIFMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T122219 NZ_CP038381:c26346-26086 [Escherichia coli O157:H7]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACATTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAATCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTATTTTTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT122219 WP_001132407.1 NZ_CP038381:c26096-25845 [Escherichia coli O157:H7]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT122219 NZ_CP038381:c26096-25845 [Escherichia coli O157:H7]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A3E1XBA7