122214

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4665065..4665615 Replicon chromosome
Accession NZ_CP038380
Organism Escherichia coli O157:H7 strain E32511

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag E3170_RS23340 Protein ID WP_001160975.1
Coordinates 4665065..4665379 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag E3170_RS23345 Protein ID WP_000125566.1
Coordinates 4665382..4665615 (-) Length 78 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3170_RS23315 4660740..4662026 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
E3170_RS23320 4662026..4663015 + 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
E3170_RS23325 4663012..4663833 + 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
E3170_RS23330 4663836..4664213 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
E3170_RS23335 4664220..4665068 + 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
E3170_RS23340 4665065..4665379 - 315 WP_001160975.1 CcdB family protein Toxin
E3170_RS23345 4665382..4665615 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
E3170_RS23350 4665701..4666180 - 480 WP_000624372.1 type 3 dihydrofolate reductase -
E3170_RS23355 4666372..4668234 - 1863 WP_000377184.1 glutathione-regulated potassium-efflux system protein KefC -
E3170_RS23360 4668227..4668757 - 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
E3170_RS23365 4668865..4670196 - 1332 WP_001183177.1 MFS transporter -
E3170_RS23370 4670254..4670541 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-103)

Antitoxin

(8-77)


Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T122214 WP_001160975.1 NZ_CP038380:c4665379-4665065 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T122214 NZ_CP038380:c4665379-4665065 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT122214 WP_000125566.1 NZ_CP038380:c4665615-4665382 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT122214 NZ_CP038380:c4665615-4665382 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References