Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2585782..2586253 Replicon chromosome
Accession NZ_CP038380
Organism Escherichia coli O157:H7 strain E32511

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag E3170_RS13010 Protein ID WP_001303511.1
Coordinates 2585782..2586060 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag E3170_RS13015 Protein ID WP_001302048.1
Coordinates 2586062..2586253 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3170_RS12980 2582718..2583803 - 1086 Protein_2537 exonuclease VIII -
E3170_RS12985 2583896..2584087 - 192 WP_001090200.1 DUF1482 family protein -
E3170_RS12990 2584084..2584272 - 189 WP_000449192.1 cell division inhibition protein DicB -
E3170_RS12995 2584673..2584837 + 165 WP_001331716.1 hypothetical protein -
E3170_RS13000 2584841..2585059 - 219 WP_001171930.1 hypothetical protein -
E3170_RS13005 2585131..2585430 - 300 WP_001240334.1 hypothetical protein -
E3170_RS13010 2585782..2586060 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3170_RS13015 2586062..2586253 - 192 WP_001302048.1 hypothetical protein Antitoxin
E3170_RS13020 2586274..2586645 - 372 WP_001169686.1 hypothetical protein -
E3170_RS13025 2586867..2588080 + 1214 WP_162829202.1 IS3 family transposase -
E3170_RS13030 2588125..2588358 + 234 Protein_2547 transcriptional regulator -
E3170_RS13035 2588355..2588780 + 426 WP_000693943.1 Rha family transcriptional regulator -
E3170_RS13040 2588803..2589765 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
E3170_RS13045 2589772..2590512 + 741 WP_000788939.1 ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2569590..2629553 59963
- inside IScluster/Tn - - 2581454..2588080 6626


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T122206 WP_001303511.1 NZ_CP038380:c2586060-2585782 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T122206 NZ_CP038380:c2586060-2585782 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT122206 WP_001302048.1 NZ_CP038380:c2586253-2586062 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT122206 NZ_CP038380:c2586253-2586062 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References