Detailed information of TA system TA122168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2962431..2962902
• Replicon: chromosome
• Accession: NZ_CP038376
• Organism: Escherichia coli O157:H7 strain F1273

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E3159_RS14640
• Protein ID: WP_001303511.1
• Coordinates: 2962624..2962902 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E3159_RS14635
• Protein ID: WP_001302048.1
• Coordinates: 2962431..2962622 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3159_RS14595	2957638..2958222	-	585	WP_000206793.1	DUF551 domain-containing protein	-
E3159_RS14600	2958279..2958674	-	396	WP_001118159.1	DUF977 family protein	-
E3159_RS14605	2958690..2959459	-	770	Protein_2843	DUF1627 domain-containing protein	-
E3159_RS14610	2959485..2960225	-	741	WP_000788936.1	ATP-binding protein	-
E3159_RS14615	2960232..2961194	-	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
E3159_RS14620	2961217..2961642	-	426	WP_000693943.1	Rha family transcriptional regulator	-
E3159_RS14625	2961639..2961941	-	303	WP_000172738.1	transcriptional regulator	-
E3159_RS14630	2962039..2962410	+	372	WP_001169686.1	hypothetical protein	-
E3159_RS14635	2962431..2962622	+	192	WP_001302048.1	hypothetical protein	Antitoxin
E3159_RS14640	2962624..2962902	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3159_RS14645	2963255..2963554	+	300	WP_001240334.1	hypothetical protein	-
E3159_RS14650	2963626..2963844	+	219	WP_001171930.1	hypothetical protein	-
E3159_RS14655	2963848..2964012	-	165	WP_001331716.1	hypothetical protein	-
E3159_RS14660	2964413..2964601	+	189	WP_000449192.1	cell division inhibition protein DicB	-
E3159_RS14665	2964598..2964789	+	192	WP_001090200.1	DUF1482 family protein	-
E3159_RS14670	2964882..2967353	+	2472	WP_000048458.1	exonuclease	-
E3159_RS14675	2967426..2967677	+	252	WP_000005552.1	excisionase family protein	-
E3159_RS14680	2967712..2967896	+	185	Protein_2858	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2925086..2971189	46103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T122168 WP_001303511.1 NZ_CP038376:2962624-2962902 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T122168 NZ_CP038376:2962624-2962902 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT122168 WP_001302048.1 NZ_CP038376:2962431-2962622 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT122168 NZ_CP038376:2962431-2962622 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0