Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 2136890..2137685 | Replicon | chromosome |
| Accession | NZ_CP038374 | ||
| Organism | Escherichia coli O157:H7 strain F3113 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | B7UP43 |
| Locus tag | E3160_RS09945 | Protein ID | WP_000854914.1 |
| Coordinates | 2137311..2137685 (+) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A0B0VS41 |
| Locus tag | E3160_RS09940 | Protein ID | WP_001280954.1 |
| Coordinates | 2136890..2137264 (+) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E3160_RS09910 | 2132245..2133150 | + | 906 | WP_000203541.1 | chemotaxis protein | - |
| E3160_RS09915 | 2133147..2134217 | + | 1071 | WP_000102669.1 | patatin-like phospholipase family protein | - |
| E3160_RS09920 | 2134557..2135375 | + | 819 | WP_001234682.1 | DUF945 domain-containing protein | - |
| E3160_RS09925 | 2135466..2135951 | + | 486 | WP_000214388.1 | antirestriction protein | - |
| E3160_RS09930 | 2135967..2136443 | + | 477 | WP_001475628.1 | RadC family protein | - |
| E3160_RS09935 | 2136506..2136727 | + | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| E3160_RS09940 | 2136890..2137264 | + | 375 | WP_001280954.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| E3160_RS09945 | 2137311..2137685 | + | 375 | WP_000854914.1 | TA system toxin CbtA family protein | Toxin |
| E3160_RS09950 | 2137682..2138173 | + | 492 | WP_000976857.1 | hypothetical protein | - |
| E3160_RS09955 | 2138185..2138382 | + | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| E3160_RS09960 | 2138467..2139309 | + | 843 | WP_001280481.1 | DUF4942 domain-containing protein | - |
| E3160_RS09970 | 2139780..2140718 | + | 939 | WP_000351284.1 | glyoxylate/hydroxypyruvate reductase GhrA | - |
| E3160_RS09975 | 2140773..2141510 | + | 738 | WP_000283664.1 | zinc-binding phosphatase | - |
| E3160_RS09980 | 2141534..2142088 | + | 555 | WP_001001917.1 | molecular chaperone YcdY | - |
| E3160_RS09985 | 2142190..2142681 | + | 492 | WP_001301587.1 | DUF1097 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | iroB / csgG / csgG / csgF / csgE / csgD / csgB / csgA / csgC | 2116225..2150699 | 34474 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14117.17 Da Isoelectric Point: 7.7761
>T122124 WP_000854914.1 NZ_CP038374:2137311-2137685 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T122124 NZ_CP038374:2137311-2137685 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13718.51 Da Isoelectric Point: 6.6249
>AT122124 WP_001280954.1 NZ_CP038374:2136890-2137264 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT122124 NZ_CP038374:2136890-2137264 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LXR5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0B0VS41 |