Detailed information of TA system TA122065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2608773..2609244
• Replicon: chromosome
• Accession: NZ_CP038369
• Organism: Escherichia coli O157:H7 strain F6321

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E3162_RS13200
• Protein ID: WP_001303511.1
• Coordinates: 2608773..2609051 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E3162_RS13205
• Protein ID: WP_001302048.1
• Coordinates: 2609053..2609244 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3162_RS13160	2603779..2603963	-	185	Protein_2573	DUF3596 domain-containing protein	-
E3162_RS13165	2603998..2604249	-	252	WP_000005552.1	excisionase family protein	-
E3162_RS13170	2604322..2606793	-	2472	WP_000048458.1	exonuclease	-
E3162_RS13175	2606886..2607077	-	192	WP_001090200.1	DUF1482 family protein	-
E3162_RS13180	2607074..2607262	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E3162_RS13185	2607663..2607827	+	165	WP_001331716.1	hypothetical protein	-
E3162_RS13190	2607831..2608049	-	219	WP_001171930.1	hypothetical protein	-
E3162_RS13195	2608121..2608420	-	300	WP_001240334.1	hypothetical protein	-
E3162_RS13200	2608773..2609051	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3162_RS13205	2609053..2609244	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E3162_RS13210	2609265..2609636	-	372	WP_001169686.1	hypothetical protein	-
E3162_RS13215	2609734..2610036	+	303	WP_000172738.1	transcriptional regulator	-
E3162_RS13220	2610033..2610458	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E3162_RS13225	2610481..2611443	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E3162_RS13230	2611450..2612190	+	741	WP_000788938.1	ATP-binding protein	-
E3162_RS13235	2612216..2612985	+	770	Protein_2588	DUF1627 domain-containing protein	-
E3162_RS13240	2613001..2613396	+	396	WP_001118159.1	DUF977 family protein	-
E3162_RS13245	2613453..2614037	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2601612..2646584	44972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T122065 WP_001303511.1 NZ_CP038369:c2609051-2608773 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T122065 NZ_CP038369:c2609051-2608773 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT122065 WP_001302048.1 NZ_CP038369:c2609244-2609053 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT122065 NZ_CP038369:c2609244-2609053 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0