Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 28681..29206 Replicon plasmid pF6667-1
Accession NZ_CP038368
Organism Escherichia coli O157:H7 strain F6667

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag E4U72_RS27685 Protein ID WP_001159868.1
Coordinates 28901..29206 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag E4U72_RS27680 Protein ID WP_000813634.1
Coordinates 28681..28899 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U72_RS27650 23964..24194 + 231 WP_010891288.1 hypothetical protein -
E4U72_RS27655 24315..25055 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
E4U72_RS27660 25340..26317 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
E4U72_RS27665 26725..26925 - 201 WP_000708307.1 hypothetical protein -
E4U72_RS27670 26922..27542 - 621 WP_001248529.1 hypothetical protein -
E4U72_RS27675 27539..28222 - 684 WP_010891291.1 DNA-binding protein -
E4U72_RS27680 28681..28899 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E4U72_RS27685 28901..29206 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
E4U72_RS27690 29207..30013 + 807 WP_000016989.1 site-specific integrase -
E4U72_RS27700 32025..32780 + 756 WP_000852148.1 replication initiation protein RepE -
E4U72_RS27705 32931..34144 + 1214 WP_162829202.1 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP 1..93665 93665


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T122047 WP_001159868.1 NZ_CP038368:28901-29206 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T122047 NZ_CP038368:28901-29206 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT122047 WP_000813634.1 NZ_CP038368:28681-28899 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT122047 NZ_CP038368:28681-28899 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References