122046

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/Xre(antitoxin)
Location 20825..21323 Replicon plasmid pF6667-2
Accession NZ_CP038367
Organism Escherichia coli O157:H7 strain F6667

Toxin (Protein)


Gene name TsxA Uniprot ID A0A0K6A0X9
Locus tag E4U72_RS27455 Protein ID WP_000702249.1
Coordinates 21057..21323 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID A0A3H7VMJ5
Locus tag E4U72_RS27450 Protein ID WP_001450803.1
Coordinates 20825..21067 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U72_RS27410 16046..16384 - 339 WP_000753495.1 hypothetical protein -
E4U72_RS27415 16730..17137 + 408 WP_001256557.1 hypothetical protein -
E4U72_RS27420 17147..17908 + 762 WP_001097937.1 conjugal transfer protein TraL -
E4U72_RS27425 17905..18609 + 705 WP_001279865.1 hypothetical protein -
E4U72_RS27430 18638..19075 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin -
E4U72_RS27435 19100..19282 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin -
E4U72_RS27440 19721..19998 - 278 Protein_23 cobalamin biosynthesis protein CbiX -
E4U72_RS27445 20056..20385 - 330 WP_000449468.1 hypothetical protein -
E4U72_RS27450 20825..21067 - 243 WP_001450803.1 hypothetical protein Antitoxin
E4U72_RS27455 21057..21323 - 267 WP_000702249.1 hypothetical protein Toxin
E4U72_RS27460 21374..21598 - 225 WP_000749828.1 hypothetical protein -
E4U72_RS27465 21694..21918 - 225 WP_000866040.1 hypothetical protein -
E4U72_RS27470 21959..22282 - 324 WP_000432877.1 hypothetical protein -
E4U72_RS27475 23960..24964 + 1005 WP_001208090.1 replication initiation protein -
E4U72_RS27480 25382..25720 - 339 WP_000612441.1 helix-turn-helix domain-containing protein -
E4U72_RS27485 25919..26206 + 288 WP_000332249.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..39233 39233


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10242.76 Da        Isoelectric Point: 9.9280

>T122046 WP_000702249.1 NZ_CP038367:c21323-21057 [Escherichia coli O157:H7]
MKIRKGDRQYYLNKEGNMFHLVKRVKTFSKSATLGKTKATVITVADLIFHEDAFDTIDFTRGGLRENDKEVVSMMIQEMN
NEREKNVN

Download         Length: 267 bp

>T122046 NZ_CP038367:c21323-21057 [Escherichia coli O157:H7]
ATGAAGATACGAAAAGGTGACAGGCAATATTATTTGAATAAAGAAGGTAATATGTTTCATCTTGTGAAACGGGTGAAGAC
CTTTTCTAAATCTGCGACTTTAGGGAAAACAAAAGCGACAGTGATAACTGTTGCCGATCTAATTTTTCATGAAGATGCAT
TCGACACTATCGACTTTACCAGAGGCGGGTTAAGAGAGAACGACAAAGAAGTTGTTTCTATGATGATTCAGGAAATGAAC
AATGAACGAGAAAAAAATGTTAACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9288.69 Da        Isoelectric Point: 9.7120

>AT122046 WP_001450803.1 NZ_CP038367:c21067-20825 [Escherichia coli O157:H7]
MLTENTPENIKRLRQKIGLTQRECAEIFSMNPRTWRRKEEPAGTASGTALTPVEFKYLQLLAGEHPEYVLRKRDKPKCSE

Download         Length: 243 bp

>AT122046 NZ_CP038367:c21067-20825 [Escherichia coli O157:H7]
ATGTTAACTGAAAATACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACGCAAAGAGAATGTGCTGAAAT
ATTCAGCATGAACCCGCGAACCTGGCGTCGCAAGGAAGAACCTGCCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATATCTTCAGCTTTTAGCTGGGGAACATCCTGAATACGTCCTGCGCAAAAGGGATAAACCTAAATGTAGTGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K6A0X9

Antitoxin

Source ID Structure
AlphaFold DB A0A3H7VMJ5

References