Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 18638..19282 Replicon plasmid pF6667-2
Accession NZ_CP038367
Organism Escherichia coli O157:H7 strain F6667

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag E4U72_RS27435 Protein ID WP_000833471.1
Coordinates 19100..19282 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag E4U72_RS27430 Protein ID WP_000466317.1
Coordinates 18638..19075 (-) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U72_RS27410 16046..16384 - 339 WP_000753495.1 hypothetical protein -
E4U72_RS27415 16730..17137 + 408 WP_001256557.1 hypothetical protein -
E4U72_RS27420 17147..17908 + 762 WP_001097937.1 conjugal transfer protein TraL -
E4U72_RS27425 17905..18609 + 705 WP_001279865.1 hypothetical protein -
E4U72_RS27430 18638..19075 - 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4U72_RS27435 19100..19282 - 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
E4U72_RS27440 19721..19998 - 278 Protein_23 cobalamin biosynthesis protein CbiX -
E4U72_RS27445 20056..20385 - 330 WP_000449468.1 hypothetical protein -
E4U72_RS27450 20825..21067 - 243 WP_001450803.1 hypothetical protein -
E4U72_RS27455 21057..21323 - 267 WP_000702249.1 hypothetical protein -
E4U72_RS27460 21374..21598 - 225 WP_000749828.1 hypothetical protein -
E4U72_RS27465 21694..21918 - 225 WP_000866040.1 hypothetical protein -
E4U72_RS27470 21959..22282 - 324 WP_000432877.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..39233 39233


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T122045 WP_000833471.1 NZ_CP038367:c19282-19100 [Escherichia coli O157:H7]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T122045 NZ_CP038367:c19282-19100 [Escherichia coli O157:H7]
ATGAAATCTGCTGACCTGTTGAAAGAACTGATAGCTGCTGGTTGTGAGTTGAAGCGGCATAAGGCGAGCAGCCACCAGAT
ATGGTGGTCGCCCATAACAGGAAAAACTTTTCCTGTGCCCCATCCGAAAAAAGACTTGCCACTGGGTACTGTCAGATCGA
TCAGAAAAATGGCGGGGATTTAA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT122045 WP_000466317.1 NZ_CP038367:c19075-18638 [Escherichia coli O157:H7]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT122045 NZ_CP038367:c19075-18638 [Escherichia coli O157:H7]
ATGTTTTTTTCTGTGGGTGTCGAAACACCGAAAGATGACCATACGGCATATGGTATAACCGTTCCGGCTTTTGATCGTTT
TGATTTTGGCTGTGTCTCTGCTGCCGATACTCAATCAGAAATTCCTGTGATGGCGCGAGAAGCGATATTGGCTATCGTGG
AAGAAATGGTTTTAAGTGGCTCTTATTCTGTTGACGATATTCATGATGACGGCTGTTTAACATATGCCGCAAATCAGGAC
TATAGTCACTGCGATAGCTGGTTTGTAATTGATGTTGATTTGTCTGAAATTGAAGGTAAACAACAACGTATTAATATTGC
GTTGCCTGATGTGTTGATTCGTCGTATTGATGGATTTGTTAGAGAAAGTGGCGGTGTTTACAGAGATCGTAGCCATTTTC
TTGCTCAGGCCGCGCGGCATGAACTTTCATATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References