Detailed information of TA system TA122028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2625960..2626431
• Replicon: chromosome
• Accession: NZ_CP038366
• Organism: Escherichia coli O157:H7 strain F6667

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U72_RS13290
• Protein ID: WP_001303511.1
• Coordinates: 2625960..2626238 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U72_RS13295
• Protein ID: WP_001302048.1
• Coordinates: 2626240..2626431 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U72_RS13250	2620966..2621150	-	185	Protein_2589	DUF3596 domain-containing protein	-
E4U72_RS13255	2621185..2621436	-	252	WP_000005552.1	excisionase family protein	-
E4U72_RS13260	2621509..2623980	-	2472	WP_000048458.1	exonuclease	-
E4U72_RS13265	2624073..2624264	-	192	WP_001090200.1	DUF1482 family protein	-
E4U72_RS13270	2624261..2624449	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U72_RS13275	2624850..2625014	+	165	WP_001331716.1	hypothetical protein	-
E4U72_RS13280	2625018..2625236	-	219	WP_001171930.1	hypothetical protein	-
E4U72_RS13285	2625308..2625607	-	300	WP_001240334.1	hypothetical protein	-
E4U72_RS13290	2625960..2626238	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U72_RS13295	2626240..2626431	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U72_RS13300	2626452..2626823	-	372	WP_001169686.1	hypothetical protein	-
E4U72_RS13305	2626921..2627223	+	303	WP_000172738.1	transcriptional regulator	-
E4U72_RS13310	2627220..2627645	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U72_RS13315	2627668..2628630	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E4U72_RS13320	2628637..2629377	+	741	WP_000788938.1	ATP-binding protein	-
E4U72_RS13325	2629403..2630172	+	770	Protein_2604	DUF1627 domain-containing protein	-
E4U72_RS13330	2630188..2630583	+	396	WP_001118159.1	DUF977 family protein	-
E4U72_RS13335	2630640..2631224	+	585	WP_032165369.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2605952..2733175	127223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T122028 WP_001303511.1 NZ_CP038366:c2626238-2625960 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T122028 NZ_CP038366:c2626238-2625960 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT122028 WP_001302048.1 NZ_CP038366:c2626431-2626240 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT122028 NZ_CP038366:c2626431-2626240 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0