Detailed information of TA system TA122015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 467903..468657
• Replicon: chromosome
• Accession: NZ_CP038366
• Organism: Escherichia coli O157:H7 strain F6667

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: E4U72_RS02220
• Protein ID: WP_001301452.1
• Coordinates: 467903..468388 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: E4U72_RS02225
• Protein ID: WP_000801912.1
• Coordinates: 468379..468657 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U72_RS02200	463069..463827	+	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-
E4U72_RS02205	463809..465407	-	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
E4U72_RS02210	465595..466821	+	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
E4U72_RS02215	466825..467853	+	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
E4U72_RS02220	467903..468388	-	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
E4U72_RS02225	468379..468657	-	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
E4U72_RS02230	468724..471429	-	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T122015 WP_001301452.1 NZ_CP038366:c468388-467903 [Escherichia coli O157:H7]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T122015 NZ_CP038366:c468388-467903 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT122015 WP_000801912.1 NZ_CP038366:c468657-468379 [Escherichia coli O157:H7]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT122015 NZ_CP038366:c468657-468379 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9