Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
Location | 28682..29207 | Replicon | plasmid pF7349-1 |
Accession | NZ_CP038364 | ||
Organism | Escherichia coli O157:H7 strain F7349 |
Toxin (Protein)
Gene name | ccdB | Uniprot ID | V0SSI5 |
Locus tag | E4U70_RS27325 | Protein ID | WP_001159868.1 |
Coordinates | 28902..29207 (+) | Length | 102 a.a. |
Antitoxin (Protein)
Gene name | ccdA | Uniprot ID | S1PPD8 |
Locus tag | E4U70_RS27320 | Protein ID | WP_000813634.1 |
Coordinates | 28682..28900 (+) | Length | 73 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
E4U70_RS27290 | 23965..24195 | + | 231 | WP_010891288.1 | hypothetical protein | - |
E4U70_RS27295 | 24316..25056 | + | 741 | WP_001066920.1 | tyrosine-type recombinase/integrase | - |
E4U70_RS27300 | 25341..26318 | - | 978 | WP_000361615.1 | RepB family plasmid replication initiator protein | - |
E4U70_RS27305 | 26726..26926 | - | 201 | WP_000708307.1 | hypothetical protein | - |
E4U70_RS27310 | 26923..27543 | - | 621 | WP_001248529.1 | hypothetical protein | - |
E4U70_RS27315 | 27540..28223 | - | 684 | WP_010891291.1 | DNA-binding protein | - |
E4U70_RS27320 | 28682..28900 | + | 219 | WP_000813634.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
E4U70_RS27325 | 28902..29207 | + | 306 | WP_001159868.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
E4U70_RS27330 | 29208..30014 | + | 807 | WP_000016989.1 | site-specific integrase | - |
E4U70_RS27340 | 31912..32250 | + | 339 | WP_071525396.1 | RepB family plasmid replication initiator protein | - |
E4U70_RS27345 | 32838..34004 | + | 1167 | WP_000772446.1 | plasmid-partitioning protein SopA | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | - | stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP | 1..92700 | 92700 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11706.51 Da Isoelectric Point: 6.4674
>T122009 WP_001159868.1 NZ_CP038364:28902-29207 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T122009 NZ_CP038364:28902-29207 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.34 Da Isoelectric Point: 4.7405
>AT122009 WP_000813634.1 NZ_CP038364:28682-28900 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT122009 NZ_CP038364:28682-28900 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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