Detailed information of TA system TA122004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4723694..4724244
• Replicon: chromosome
• Accession: NZ_CP038363
• Organism: Escherichia coli O157:H7 strain F7349

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: E4U70_RS23605
• Protein ID: WP_001160975.1
• Coordinates: 4723694..4724008 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: E4U70_RS23610
• Protein ID: WP_000125566.1
• Coordinates: 4724011..4724244 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U70_RS23580	4719369..4720655	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
E4U70_RS23585	4720655..4721644	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
E4U70_RS23590	4721641..4722462	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
E4U70_RS23595	4722465..4722842	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
E4U70_RS23600	4722849..4723697	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
E4U70_RS23605	4723694..4724008	-	315	WP_001160975.1	CcdB family protein	Toxin
E4U70_RS23610	4724011..4724244	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
E4U70_RS23615	4724330..4724809	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
E4U70_RS23620	4725001..4726863	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
E4U70_RS23625	4726856..4727386	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
E4U70_RS23630	4727494..4728825	-	1332	WP_001183177.1	MFS transporter	-
E4U70_RS23635	4728883..4729170	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T122004 WP_001160975.1 NZ_CP038363:c4724008-4723694 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T122004 NZ_CP038363:c4724008-4723694 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT122004 WP_000125566.1 NZ_CP038363:c4724244-4724011 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT122004 NZ_CP038363:c4724244-4724011 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9