Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4232274..4232892 Replicon chromosome
Accession NZ_CP038363
Organism Escherichia coli O157:H7 strain F7349

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E4U70_RS21310 Protein ID WP_001291435.1
Coordinates 4232674..4232892 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E4U70_RS21305 Protein ID WP_000344800.1
Coordinates 4232274..4232648 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U70_RS21295 4227363..4228556 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
E4U70_RS21300 4228579..4231728 + 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
E4U70_RS21305 4232274..4232648 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E4U70_RS21310 4232674..4232892 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E4U70_RS21315 4233064..4233615 + 552 WP_000102553.1 maltose O-acetyltransferase -
E4U70_RS21320 4233731..4234201 + 471 WP_000136192.1 YlaC family protein -
E4U70_RS21325 4234365..4235915 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E4U70_RS21330 4235957..4236310 - 354 WP_000878140.1 DUF1428 domain-containing protein -
E4U70_RS21340 4236689..4237000 + 312 WP_000409911.1 MGMT family protein -
E4U70_RS21345 4237033..4237605 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T122001 WP_001291435.1 NZ_CP038363:4232674-4232892 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T122001 NZ_CP038363:4232674-4232892 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT122001 WP_000344800.1 NZ_CP038363:4232274-4232648 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT122001 NZ_CP038363:4232274-4232648 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References